| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.151131 |
| Chromosome: | chromosome 12 |
| Location: | 1435093 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487950 | (1 of 2) IPR000742//IPR017853 - EGF-like domain // Glycoside hydrolase superfamily | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACAATGAAACAAGCACACGCAGCCATG |
| Internal bar code: | GGGGGGCCCTCCCATGATTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1278 |
| LEAP-Seq percent confirming: | 99.1228 |
| LEAP-Seq n confirming: | 904 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGTTAAACACATGGCAG |
| Suggested primer 2: | CCATGCCGGTGCTAGTTAAT |