Insertion junction: LMJ.RY0402.151133_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.151133
Chromosome:chromosome_17
Location:3321721
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre17.g723300 sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TGTTGGGCGGCAGCGGGAACGGCTCGGGTG
Internal bar code:AGGGGGACCCGCGCCCCAAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:365
LEAP-Seq percent confirming:78.8764
LEAP-Seq n confirming:351
LEAP-Seq n nonconfirming:94
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCCACGTGACAGCAACTC
Suggested primer 2:GGGCTTGAGGCATAGTTACG