Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.151133 |
Chromosome: | chromosome_17 |
Location: | 3321721 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g723300 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGTTGGGCGGCAGCGGGAACGGCTCGGGTG |
Internal bar code: | AGGGGGACCCGCGCCCCAAGTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 365 |
LEAP-Seq percent confirming: | 78.8764 |
LEAP-Seq n confirming: | 351 |
LEAP-Seq n nonconfirming: | 94 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTCCACGTGACAGCAACTC |
Suggested primer 2: | GGGCTTGAGGCATAGTTACG |