| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151133 |
| Chromosome: | chromosome_17 |
| Location: | 3321721 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g723300 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTTGGGCGGCAGCGGGAACGGCTCGGGTG |
| Internal bar code: | AGGGGGACCCGCGCCCCAAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 365 |
| LEAP-Seq percent confirming: | 78.8764 |
| LEAP-Seq n confirming: | 351 |
| LEAP-Seq n nonconfirming: | 94 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCCACGTGACAGCAACTC |
| Suggested primer 2: | GGGCTTGAGGCATAGTTACG |