Insertion junction: LMJ.RY0402.151138_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.151138
Chromosome:chromosome 1
Location:83634
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g000550 Putative nucleotide-diphospho-sugar transferase; (1 of 6) PTHR10994//PTHR10994:SF61 - RETICULON // RETICULON-LIKE PROTEIN 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCTCAACCTGCACTCCGCATCCATCTGC
Internal bar code:TGTATAGGTTCTTGGAACGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:82
LEAP-Seq percent confirming:69.5556
LEAP-Seq n confirming:313
LEAP-Seq n nonconfirming:137
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACACCCTACACACACAC
Suggested primer 2:ATAGGCAGTGCTAGGAGGCA