Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.151143 |
Chromosome: | chromosome_10 |
Location: | 4256287 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre10.g450450 | FAP18 | Flagellar Associated Protein, serine endopeptidase | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCACGGCAAGACAGGCGCAGTGGCTTTGGA |
Internal bar code: | GAGTGCCATACGTGGAGTGGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 606 |
LEAP-Seq percent confirming: | 99.4318 |
LEAP-Seq n confirming: | 175 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TATGCACGCTTTGTACAGCC |
Suggested primer 2: | GGGTAGCACATCGGTCAAGT |