| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.151145 |
| Chromosome: | chromosome 1 |
| Location: | 3852483 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g024900 | (1 of 1) PTHR10501:SF19 - RNA-BINDING REGION RNP-1 DOMAIN-CONTAINING PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAGAGCCCGGTGAAGGGTCAAATCGAGG |
| Internal bar code: | TAATGGACTCGTACGTCATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1075 |
| LEAP-Seq percent confirming: | 39.6226 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 96 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCACCCTACGGGACTTTAC |
| Suggested primer 2: | GCCCTGCTACTTGACCTGAG |