Insertion junction: LMJ.RY0402.151149_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.151149
Chromosome:chromosome 17
Location:5607011
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g738000 AGG2 Aggregation 2; (1 of 6) PTHR15907:SF21 - DUF614 FAMILY PROTEIN 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTACAAGCCGGCAATGCACGCGCCACGG
Internal bar code:TGCAACTGGCCCGGATTAGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:882
LEAP-Seq percent confirming:89.0645
LEAP-Seq n confirming:6874
LEAP-Seq n nonconfirming:844
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCTATGATCAGAACGTCG
Suggested primer 2:ATTTCCTGCTGTTTGGTTGG