Insertion junction: LMJ.RY0402.151197_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.151197
Chromosome:chromosome 6
Location:6360146
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g292100 (1 of 1) PTHR30222//PTHR30222:SF3 - SPERMIDINE/PUTRESCINE-BINDING PERIPLASMIC PROTEIN // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCTCACGGAGCGCAATCTCGACGCGTCGA
Internal bar code:ACAAAGCTGGTGATGACGGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:311
LEAP-Seq percent confirming:99.7175
LEAP-Seq n confirming:353
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGAAGCCGTGCAACTAA
Suggested primer 2:CTAACTGAGGGGGTGATGGA