| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151202 |
| Chromosome: | chromosome 5 |
| Location: | 1282985 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245150 | (1 of 30) PF13450 - NAD(P)-binding Rossmann-like domain (NAD_binding_8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTACGCCGATCACGTGCGCACCCACTCC |
| Internal bar code: | CTTTTCACATTGGCGCTGGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 77.305 |
| LEAP-Seq n confirming: | 109 |
| LEAP-Seq n nonconfirming: | 32 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCGATGATCGTAGAGGGG |
| Suggested primer 2: | GAGTTTGTGAATGAGCGCAA |