Insertion junction: LMJ.RY0402.151204_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.151204
Chromosome:chromosome 12
Location:8286380
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g550153 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCACCCGCGCGTCTGCGCCTGCTGCTG
Internal bar code:GCGGGATCGAAGCGCGACAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:160
LEAP-Seq percent confirming:98.7104
LEAP-Seq n confirming:842
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCATACCACCAACCACCC
Suggested primer 2:ATACGCAAAGCCAATCATCC