| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.151207 |
| Chromosome: | chromosome 12 |
| Location: | 4940209 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g525600 | (1 of 8) IPR001810//IPR006553 - F-box domain // Leucine-rich repeat, cysteine-containing subtype | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGCCGGCGCCGCGCCTGCACCAGCATC |
| Internal bar code: | GCCGTAAACCATGTGGGGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 131 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 99 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCGACAGGCTGGTCAGT |
| Suggested primer 2: | CATCAGCGATACCGACATTG |