| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.151211 |
| Chromosome: | chromosome 17 |
| Location: | 3078920 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g720900 | (1 of 100) IPR001810 - F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGGCACACATCCCTCCCGCCCTCGGCCT |
| Internal bar code: | TAGCTCCGTTCTAGAGTTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 173 |
| LEAP-Seq percent confirming: | 99.659 |
| LEAP-Seq n confirming: | 2630 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCCTAGTAGGGCTAGGTG |
| Suggested primer 2: | CTGGAACTCGTCTCGGTCTC |