| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151222 |
| Chromosome: | chromosome 1 |
| Location: | 2719819 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016150 | (1 of 1) PTHR16222//PTHR16222:SF15 - ADP-RIBOSYLGLYCOHYDROLASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGAGTATTGCTGCGGGGTGGGGTGGGC |
| Internal bar code: | CACGCTCTGCACGTGGAAGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 653 |
| LEAP-Seq percent confirming: | 67.0 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGTCTACCTTCTCCAGCG |
| Suggested primer 2: | CAGCAGGGACCTTACAGAGC |