| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.151228 |
| Chromosome: | chromosome 5 |
| Location: | 1300052 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245101 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTGCCGGTGCCTCGACCGCATTGTGTC |
| Internal bar code: | GTGGTAGCGTGCGACGATATTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 812 |
| LEAP-Seq percent confirming: | 99.8868 |
| LEAP-Seq n confirming: | 3530 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGGCTGACCATGCTACTG |
| Suggested primer 2: | AAGGAGGGAAGCGAGGTTAG |