| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151248 |
| Chromosome: | chromosome 10 |
| Location: | 4036139 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449200 | ANK5 | (1 of 1) PTHR22849//PTHR24133//PTHR24133:SF23 - WDSAM1 PROTEIN // FAMILY NOT NAMED // SUBFAMILY NOT NAMED; Predicted protein with ankyrin repeats | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACTGCCATGTACATCGCACCATCTCGC |
| Internal bar code: | GCCCCGTATTGTGGGCTCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1 |
| LEAP-Seq percent confirming: | 98.9583 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGCCATCCGTAGTTTCG |
| Suggested primer 2: | AATTTTGCTTTGCAACCGAC |