| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.151255 |
| Chromosome: | chromosome 12 |
| Location: | 7590164 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555100 | (1 of 726) IPR011009 - Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTGCGGCTCATCAAGAAGCGCCTGCCGG |
| Internal bar code: | TACAGTAAGAATAGGGCATGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 381 |
| LEAP-Seq percent confirming: | 99.7667 |
| LEAP-Seq n confirming: | 2566 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCGCTGACAGCAGGATTAT |
| Suggested primer 2: | GAAGCCATAGCCAATTCTCG |