| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.151270 |
| Chromosome: | chromosome 17 |
| Location: | 2274528 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713700 | IAD2 | Indoleamine 2%252C3-dioxygenase; (1 of 1) 1.13.11.52 - Indoleamine 2,3-dioxygenase / Tryptophan pyrrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCTTGAATACCGGTAAGTTGAATGCTGAT |
| Internal bar code: | CACGAGGAACTTCCGCGGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 67 |
| LEAP-Seq percent confirming: | 99.4983 |
| LEAP-Seq n confirming: | 595 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCCCATACCTCTCAGGACC |
| Suggested primer 2: | TTCATGTCGACAAGCTGAGG |