Insertion junction: LMJ.RY0402.151279_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.151279
Chromosome:chromosome 6
Location:1696861
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g261350 (1 of 1) PF06108 - Protein of unknown function (DUF952) (DUF952) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGACGGTCCTGTGACATCCCCCGTCCAGT
Internal bar code:CGATGTCTCTTAGAAGGGTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:484
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1194
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GACTGCTGCTGTGGTGTCAT
Suggested primer 2:GAACTCTTTGCTGCGGTTTC