| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151282 |
| Chromosome: | chromosome 12 |
| Location: | 6975779 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560050 | ERM11,ERM12,RVT1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGGAGGAGGATGGAGCGGCAGGGCGGG |
| Internal bar code: | GTCTGCGGCCCATGCCACGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1041 |
| LEAP-Seq percent confirming: | 79.302 |
| LEAP-Seq n confirming: | 5885 |
| LEAP-Seq n nonconfirming: | 1536 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | TTTGAAAGCGCAAACACAAC |