| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.151315 |
| Chromosome: | chromosome 10 |
| Location: | 1157103 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425800 | ORN1 | (1 of 1) 3.1.13.3 - Oligonucleotidase; Putative organellar oligoribonuclease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCTCGGTTGTGGGCGGTATGCCCAGGGT |
| Internal bar code: | TACGCATGCTCTGTAGCTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 86.8644 |
| LEAP-Seq n confirming: | 205 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGCGGCTTAGTCCGTCT |
| Suggested primer 2: | GCTGTTGTCTCCTCCCTCTG |