| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.151323 |
| Chromosome: | chromosome 2 |
| Location: | 6941298 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g120100 | RBCS1 | (1 of 2) K01602 - ribulose-bisphosphate carboxylase small chain (rbcS); Ribulose-1%252C5-bisphosphate carboxylase/oxygenase small subunit 1, chloroplastic | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATACCGCAGCCAGCTTCCGTCAGCCAGCC |
| Internal bar code: | CCCGGGAGAGGTTATCGGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1177 |
| LEAP-Seq percent confirming: | 76.1806 |
| LEAP-Seq n confirming: | 1468 |
| LEAP-Seq n nonconfirming: | 459 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTTTGGAAAAGTTGCTGC |
| Suggested primer 2: | TACTCGGTATCCCTTGGTGC |