| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.151344 |
| Chromosome: | chromosome 1 |
| Location: | 4531893 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030900 | (1 of 1) 6.2.1.26 - o-succinylbenzoate--CoA ligase / OSB-CoA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCGAAGGAGGAAGTGTAGCCGGCCCGAG |
| Internal bar code: | AACTGTGCGCGCGCCGTATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 923 |
| LEAP-Seq percent confirming: | 99.4902 |
| LEAP-Seq n confirming: | 1366 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAGCACGTGAAGGCTGAC |
| Suggested primer 2: | GGCCGATGAAGACATGAAGT |