| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151358 |
| Chromosome: | chromosome 3 |
| Location: | 9022790 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g210065 | (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGATAGCCCTTGCTCCTGGGCTCGGCTAAG |
| Internal bar code: | CGTCCGCCAAAGAGACGGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 191 |
| LEAP-Seq percent confirming: | 85.9531 |
| LEAP-Seq n confirming: | 2674 |
| LEAP-Seq n nonconfirming: | 437 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTTGAAGTCCATCCGCAG |
| Suggested primer 2: | CGTGCCCACCTACCACTACT |