| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151398 |
| Chromosome: | scaffold 19 |
| Location: | 21629 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre19.g750197 | (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGACCGAGCAGCACGAGAGCCCCTCAAA |
| Internal bar code: | GCAATTCCCTGCCTCTCTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 859 |
| LEAP-Seq percent confirming: | 94.5736 |
| LEAP-Seq n confirming: | 122 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTTTACACGTTGGACGTT |
| Suggested primer 2: | GGCAGATGGAAGAAAAGCAG |