| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151458 |
| Chromosome: | chromosome 7 |
| Location: | 413329 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315050 | GGH1 | (1 of 2) 3.4.19.9 - Gamma-glutamyl hydrolase / Pteroyl-poly-alpha-glutamate hydrolase; Gamma-glutamyl hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTGCCCCACCCGCCTACCAGCCGTTCGG |
| Internal bar code: | AGTTAGGGTTAAGCCCACGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 100 |
| LEAP-Seq percent confirming: | 98.5075 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACAGCTTTGCTCGCTCT |
| Suggested primer 2: | TGAACAGAGGGAAGAAGCGT |