Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.151463 |
Chromosome: | chromosome_17 |
Location: | 762460 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g701450 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGCCTCAGCCGACTCGGGCCCCCTCGCAGC |
Internal bar code: | GGTCTTCTGTTAGTCGTCTGGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 497 |
LEAP-Seq percent confirming: | 98.8552 |
LEAP-Seq n confirming: | 1727 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTTTTTGGTTGCATCGGTA |
Suggested primer 2: | GCTGCTAAAGGCCAGTTCC |