| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.151501 |
| Chromosome: | chromosome 9 |
| Location: | 2929072 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388000 | CDKD1 | CDK activating kinase; (1 of 1) K02202 - cyclin-dependent kinase 7 (CDK7) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCAGCTCATCAGCACAGCGCGGCACCCA |
| Internal bar code: | GGTCGGAGGTGCCTGGCTAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1116 |
| LEAP-Seq percent confirming: | 99.7292 |
| LEAP-Seq n confirming: | 1473 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACACTTCGCAACGACTTC |
| Suggested primer 2: | TGGGGTAGATATGGCTGAGG |