Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.151509 |
Chromosome: | chromosome_12 |
Location: | 7113174 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g559250 | FTT1 | 14-3-3 protein | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCAGGTCGCTTTTGGCGCGTATGCCGCGAC |
Internal bar code: | AGGCAACGGAGCTGGTAGTCGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 840 |
LEAP-Seq percent confirming: | 84.4398 |
LEAP-Seq n confirming: | 1221 |
LEAP-Seq n nonconfirming: | 225 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGTCTTAAACTCAGCCAGG |
Suggested primer 2: | GTACACTTGGGTGTGCGATG |