| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151511 |
| Chromosome: | chromosome_1 |
| Location: | 4391616 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g029750 | FAK2,FAK1,DAAK1 | ODA5-associated flagellar adenylate kinase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCTGTGAAGGGAGCATGGATTAAAAATGT |
| Internal bar code: | GATTGTGAGGCCGGCTGAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 7 |
| LEAP-Seq percent confirming: | 97.8836 |
| LEAP-Seq n confirming: | 185 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGCCTAGCCTCACAAAC |
| Suggested primer 2: | GCGATCAGATCAAGGAGGAG |