| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.151522 |
| Chromosome: | chromosome 6 |
| Location: | 3250517 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g276500 | HTA23 | Histone H2A | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTAGCCGGGACACGATGCGGACTCTCGCC |
| Internal bar code: | CGCGAGTCCTCGCCCCCGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 454 |
| LEAP-Seq percent confirming: | 98.5646 |
| LEAP-Seq n confirming: | 618 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAGAAGGGCAAGTACGC |
| Suggested primer 2: | ATGTGAAGGGGTACGAGTGC |