| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.151536 |
| Chromosome: | chromosome 1 |
| Location: | 7235415 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g052100 | PRPL18 | (1 of 1) K02881 - large subunit ribosomal protein L18 (RP-L18, MRPL18, rplR); Chloroplast ribosomal protein L18 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCCTCCGCAAAAAGGTGACATATTATG |
| Internal bar code: | TGATTTGGGCTTTGCGGAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 138 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 279 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTCTCAATGTTGTGGGC |
| Suggested primer 2: | GCCGCCTAGAGTTGACTACG |