| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.151544 |
| Chromosome: | chromosome 8 |
| Location: | 2161734 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g369350 | (1 of 2) 2.6.1.52 - Phosphoserine transaminase / PSAT | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTATAAACTCAACGCACATACCGATGCTC |
| Internal bar code: | CCGCAACGCTCGGTAAGCATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 98.9924 |
| LEAP-Seq n confirming: | 393 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGAGCTTGGGCACCTACA |
| Suggested primer 2: | CATTACCTCGGTCTCACCGT |