Insertion junction: LMJ.RY0402.151582_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.151582
Chromosome:chromosome 2
Location:4401604
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g099700 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAAGACAGGTGGATACATGGTGGATCGTG
Internal bar code:GGGCGTCGTTGGTTGATCACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:11
LEAP-Seq percent confirming:98.9247
LEAP-Seq n confirming:92
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AACCCACCATACTGCTCCAG
Suggested primer 2:TGACGCACATTCCCTAATCA