| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.151607 |
| Chromosome: | chromosome 3 |
| Location: | 3944114 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171600 | XPP1,XPP | Exopolyphosphatase; (1 of 2) K03787 - 5'-nucleotidase (surE) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTCGCTGCCTGCACTTGCCTGCCGCCAT |
| Internal bar code: | GGTCATGGTGATGCACAGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 491 |
| LEAP-Seq percent confirming: | 99.5343 |
| LEAP-Seq n confirming: | 2565 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTACACGAGCGATCTAGG |
| Suggested primer 2: | ATTTCCTGACTCATGCCCAC |