| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151614 |
| Chromosome: | chromosome 10 |
| Location: | 2415834 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435600 | TMEM17 | Transmembrane protein 17; (1 of 1) K19384 - transmembrane protein 17 (TMEM17) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGGATGTGAGGTGCGTGATGCGGACTC |
| Internal bar code: | CTGCAAGGGGTAACGGCGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTCGGATTCCTCATTTGT |
| Suggested primer 2: | CTCATCATGTTCACGCTGCT |