Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.151631 |
Chromosome: | chromosome 3 |
Location: | 4046098 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g172350 | (1 of 1) PF14736 - Protein N-terminal asparagine amidohydrolase (N_Asn_amidohyd) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTGCCTCTGCCTGCGCCCCGCTAGTATG |
Internal bar code: | GGTTCAGTTCGCAGGTAAAGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 672 |
LEAP-Seq percent confirming: | 99.6564 |
LEAP-Seq n confirming: | 290 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTTCTTCCCCTTCGTTTTCC |
Suggested primer 2: | TGGTGTCTGCAACGATTAGC |