Insertion junction: LMJ.RY0402.151644_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.151644
Chromosome:chromosome 17
Location:5149009
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g735375 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACACACAACAGGCAAATCCTCTCCTCCCT
Internal bar code:CCGGCGATTTCAGAGACAATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:819
LEAP-Seq percent confirming:99.7487
LEAP-Seq n confirming:1191
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGACTAGCTCCACAACCC
Suggested primer 2:AGGCCGTCAAATTACAAACG