| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.151661 |
| Chromosome: | chromosome 1 |
| Location: | 4633978 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g031875 | (1 of 3) 4.4.1.15 - D-cysteine desulfhydrase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCTAGTGATTGAGTGGTGTGCTGGTGTA |
| Internal bar code: | TGACTGAGTTGTTGACACTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 89.8148 |
| LEAP-Seq n confirming: | 97 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGTGATGGTCAGGGGAG |
| Suggested primer 2: | TTTGCCAGAAGCAGGAGTTT |