| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.151678 |
| Chromosome: | chromosome 17 |
| Location: | 620065 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700166 | (1 of 5) K08867 - WNK lysine deficient protein kinase [EC:2.7.11.1] (WNK, PRKWNK) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCCCCCTCACGTCATGATGTTCTTGTG |
| Internal bar code: | TCTTGGACATTGGTTAGCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 8 |
| LEAP-Seq percent confirming: | 99.2537 |
| LEAP-Seq n confirming: | 133 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCTGCAGTACAACATCCT |
| Suggested primer 2: | GTATCCTCCGCTGTGCTCAT |