| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.151682 |
| Chromosome: | chromosome 3 |
| Location: | 6256018 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192750 | (1 of 4) 2.7.11.13 - Protein kinase C / PKC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGAACCCCCGAACCCGAACCAATTCCC |
| Internal bar code: | CCTGGTTAGACCCGTCTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 371 |
| LEAP-Seq percent confirming: | 93.4343 |
| LEAP-Seq n confirming: | 555 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGACGGTGCTCAGATTG |
| Suggested primer 2: | GCACACGGTGAGATAGGGAT |