| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.151682 |
| Chromosome: | chromosome 3 |
| Location: | 6256034 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192750 | (1 of 4) 2.7.11.13 - Protein kinase C / PKC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGACGGGGGGCTTGTCCGATCGTGGGAA |
| Internal bar code: | TAATGCTAAGAGATCGAATTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 94 |
| LEAP-Seq percent confirming: | 98.7421 |
| LEAP-Seq n confirming: | 157 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGACGGTGCTCAGATTG |
| Suggested primer 2: | GCACACGGTGAGATAGGGAT |