| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.151715 |
| Chromosome: | chromosome 13 |
| Location: | 2182752 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g578201 | RRM13 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGATGGGGCGTGGGATGGGGCTTACGAG |
| Internal bar code: | CCATCGTCGGGGGGACCGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 97.2656 |
| LEAP-Seq n confirming: | 3735 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCTGCTACGATACCTGC |
| Suggested primer 2: | AACAATTGGGTGAAAGTCGC |