Insertion junction: LMJ.RY0402.151721_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.151721
Chromosome:chromosome 11
Location:2149034
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g469500 FXL6 FixL-like PAS domain protein; (1 of 12) 2.7.13.3 - Histidine kinase / Protein kinase (histidine) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGCAACCAGGCACTACAAGGGAGCGAAC
Internal bar code:TTCCTAATCGCGACTTTTTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:324
LEAP-Seq percent confirming:37.8274
LEAP-Seq n confirming:1473
LEAP-Seq n nonconfirming:2421
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCTCGCCTTACACAATAA
Suggested primer 2:TTGGACACCACGAACTGGTA