| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.151728 |
| Chromosome: | chromosome 13 |
| Location: | 1585672 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573250 | (1 of 7) 2.8.1.1 - Thiosulfate sulfurtransferase / Thiosulfate thiotransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCCGCTTCACAACAGCCAGAGCTTATG |
| Internal bar code: | CATGGACAGGCCGGATCTGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 612 |
| LEAP-Seq percent confirming: | 99.6288 |
| LEAP-Seq n confirming: | 1342 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAGTAGAGCTTTGGCTGGG |
| Suggested primer 2: | AGATGTGTCTGGGGGAACTG |