| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151731 |
| Chromosome: | chromosome 7 |
| Location: | 71135 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g312580 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCCCGCCTCCCTCGCCGTGCGAGGTTG |
| Internal bar code: | CCCTCCTGCAAAGTTGAGTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1113 |
| LEAP-Seq percent confirming: | 58.6086 |
| LEAP-Seq n confirming: | 834 |
| LEAP-Seq n nonconfirming: | 589 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTGAAATGGGAGAATCAT |
| Suggested primer 2: | GGCAGTTTGGTCAGGTTTGT |