| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151790 |
| Chromosome: | chromosome 1 |
| Location: | 5764573 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041150 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATAGGCTCGCGCCTCAGCCGACGCCCCT |
| Internal bar code: | GCGAATAGGATGGTACGGTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 332 |
| LEAP-Seq percent confirming: | 82.4351 |
| LEAP-Seq n confirming: | 826 |
| LEAP-Seq n nonconfirming: | 176 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTCCTCCCACAACAACT |
| Suggested primer 2: | ATGGGACTGTGGAGATCAGC |