| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151798 |
| Chromosome: | chromosome_3 |
| Location: | 76245 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g143827 | FBB7 | Flagellar/basal body protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCGCCCGTCCTCACCTGCAGTCGCGACATC |
| Internal bar code: | CGGCGCCAGGAGGGAATGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1025 |
| LEAP-Seq percent confirming: | 99.8007 |
| LEAP-Seq n confirming: | 4005 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGACCTGAGGAATGCAAGC |
| Suggested primer 2: | CGAAGCAGTTGATGGTGCTA |