Insertion junction: LMJ.RY0402.151810_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.151810
Chromosome:chromosome 2
Location:4805098
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g102850 CPZ (1 of 1) IPR000834//IPR016024 - Peptidase M14, carboxypeptidase A // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGACGGGGCGCAGGAGTTCGCAGTTGCAA
Internal bar code:CTCTAGTGTGTGTCTGATTGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:821
LEAP-Seq percent confirming:99.469
LEAP-Seq n confirming:2435
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTTTCACGACAGCTTGGA
Suggested primer 2:CTGATGATGCACCTGACACC