| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.151813 |
| Chromosome: | chromosome_16 |
| Location: | 4376893 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre16.g671400 | ARS1 | Periplasmic arylsulfatase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTATACAGCTTGTTGCCCTGGTTACCAGAC |
| Internal bar code: | ACACGGCGAAGGGACGAACGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 712 |
| LEAP-Seq percent confirming: | 86.9128 |
| LEAP-Seq n confirming: | 259 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAGGAGTCACGGAGCTTCA |
| Suggested primer 2: | GATAAGAACGGGCATCAGGA |