Insertion junction: LMJ.RY0402.151820_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.151820
Chromosome:chromosome 5
Location:391693
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre05.g232950 (1 of 1) K13110 - microfibrillar-associated protein 1 (MFAP1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTTCCCCCCCCCCCCCCCCCCCCCCCC
Internal bar code:ACGTTGAGCCGTTAGTAGCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGGAGGAGTCAGACAAGG
Suggested primer 2:AATGTTTCAACCTTGCGACC