| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.151822 |
| Chromosome: | chromosome 8 |
| Location: | 137694 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358538 | (1 of 1) K06443 - lycopene beta-cyclase (lcyB, crtL1, crtY) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGGCGAAAAAATGCAGCGGCACGGCGGC |
| Internal bar code: | ATTTAGGCATCTTAGAAGTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 872 |
| LEAP-Seq percent confirming: | 98.8574 |
| LEAP-Seq n confirming: | 4499 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGCCGTGTCAACCCTAC |
| Suggested primer 2: | TGGGAGATGGAGAGGTTGAC |